• Test Code - PL0111
  • Test Name - Vitamin D-25 (Hydroxy)
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Test Details & Preparation


  • A vitamin D test is used to determine if bone weakness, bone malformation, or abnormal metabolism of calcium (reflected by abnormal calcium, phosphorus, PTH) is occurring as a result of a deficiency or excess of vitamin D.

  • A vitamin D test helps diagnose problems with parathyroid gland functioning since PTH is essential for vitamin D activation.

  • Vitamin D tests helps monitor the health status of individuals with diseases that interfere with fat absorption, such as cystic fibrosis and Crohn’s disease, since vitamin D is a fat-soluble vitamin and is absorbed from the intestine like a fat.

  • A vitamin D test helps determine the effectiveness of treatment when vitamin D, calcium, phosphorus, or magnesium supplementation is prescribed.

  • Vitamin D tests, such as 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D are performed when calcium levels in a person is either low or high leading to vitamin D deficiency or excess amounts of vitamin D respectively.

  • A blood sample is obtained by inserting a needle into a vein in the arm for Vitamin D tests.

  • No test preparation is required for Vitamin D tests.

  • A low blood level of 25-hydroxyvitamin D may mean that a person is not getting enough exposure to sunlight or enough dietary vitamin D to meet his or her body's demand.

  • A high level of 25-hydroxyvitamin D usually reflects excess supplementation from vitamin tablets or other nutritional supplements.

  • A low level of 1, 25-dihydroxyvitamin D can be seen in kidney disease and is one of the earliest changes to occur in persons with early kidney failure.

  • A high level of 1, 25-dihydroxyvitamin D may occur when there is excess parathyroid hormone or when there are diseases, such as sarcoidosis or some lymphomas that can make 1, 25-dihydroxyvitamin D outside of the kidneys.


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