HIV-1 RNA, Quantitative Real-Time PCR testing, antiretroviral drug therapy, HIV-1 infection, HIV-1 RNA" /> HIV-1 RNA, Quantitative Real-Time PCR testing, antiretroviral drug therapy, HIV-1 infection, HIV-1 RNA" />
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Test Details
Test Code:PL0606
Test Name:HIV RNA, Quantitative (RT-PCR)
Test Details & Preparation
HIV-1 RNA, Quantitative Real-Time PCR testing is done to assess prognosis, monitor progression of HIV-1 infection and monitor effect of antiretroviral drug therapy.

Measurement of HIV-1 RNA plasma levels (viral load) provides quantitative measurement of viremia and is used in conjunction with CD4+ T-cell counts. The baseline HIV RNA level, combined with the baseline CD4 count, predicts progression to AIDS and death. Periodic viral load assessment is used to track progression of the infection and is the most important indicator of antiretroviral treatment response. Stable or increasing levels are associated with disease progression and/or treatment failure, whereas decreasing levels indicate therapeutic response. The primary goal of treatment is to decrease the viral load below detectable levels, which typically occurs within 12 to 24 weeks.

Measurement of the HIV RNA level and the CD4 count is recommended at every 3 to 6 months to monitor progression of HIV infection in untreated patients 2 to 8 weeks after initiation of therapy and every 4 to 8 weeks thereafter until the viral load is below detectable limits 2 to 8 weeks after changing therapy and every 6 to 12 months to monitor continuing effectiveness of therapy in stable patients unless new treatment with interferon, corticosteroids, or anti-neoplastic agents is initiated. HIV-1 RNA testing is generally not recommended within 4 weeks of immunization or resolution of intercurrent infections.

HIV-1 RNA, Quantitative Real-Time PCR testing is recommended for individuals recently diagnosed with HIV-1 infection individuals who are in the clinically latent period of the infection and not receiving antiretroviral therapy and individuals receiving antiretroviral therapy.

A three-fold change in HIV-1 RNA viral load is considered statistically significant. Increasing levels may be due to disease progression, failed antiretroviral therapy, suboptimal adherence to treatment, immunizations, or occasionally, other active infections (e.g., tuberculosis, pneumococcal pneumonia). Decreasing levels indicate therapeutic response and improved clinical outcome.
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